Indirect Somatic Embryogenesis of Japonica Rice Through Anther Culture

Zusatztext

Time utilization was observed in 16 growth regulator treatments of callus induction and 18 growth regulator treatments of regeneration. Data of callus induction and regeneration were analyzed with factorial completely randomized design (FCRD). The highest callus induction frequency was recorded in Azucena into callus induction treatment T4 containing 2, 4-D 2 mg/l + Kinetin 1 mg/l (49.99%) followed by in Moroberekan into callus induction treatment T11 containing 2, 4-D 1 mg/l + NAA 2 mg/l + Kinetin 0.5 mg/l (48.64%) in 17 weeks and 9 weeks. The highest shoot regeneration frequency was recorded in Azucena into shoot regeneration treatment T10 containing Kinetin 0.5 mg/l + BAP 2 mg/l + NAA 1 mg/l (83.33%) in 28 days followed by Moroberekan into shoot regeneration treatment T16 containing Kinetin 2 mg/l + BAP 1 mg/l + NAA 1 mg/l (80.0%) in 35 days. The highest plantlet regeneration frequency was recorded in Moroberekan into plantlet regeneration treatment T16 containing Kinetin 2 mg/l + BAP 1 mg/l + NAA 1 mg/l (80.0%) in 35 days followed by plantlet regeneration treatment T10 containing Kinetin 0.5 mg/l + BAP 2 mg/l + NAA 1 mg/l (55.55%) in 21 days.

Autorenportrait

I am Avinash Sharma and belongs to Bihar state. I completed B.Sc. (Agriculture) from Central Agricultural University, Imphal, Manipur and studied M.Sc. (Agriculture) with specialization Agricultural Biotechnology from University of Agricultural Sciences, Bangalore. I achieved ICAR-NET and published five research articles into different journals.

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