DNA Sequencing Protocols

Methods in Molecular Biology 167

Colin A Graham/Alison J M Hill

Humana Press

InhaltsangabeIntroduction to DNA Sequencing, Colin A. Graham and Alison J. M. Hill. The Universal Primers and the Shotgun DNA Sequencing Method, Joachim Messing. M13 Sequencing, Qingzhong Yu. Primer Design and Primer-Directed Sequencing, Ulrike Gerischer and Peter Dürre. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction, Ulrike Gerischer. Solid Phase Fluorescent Sequencing of the CFTR Gene, Harry Cuppens and Jean-Jacques Cassiman. Shotgun DNA Sequencing, Alan T. Bankier. Cycle Sequencing, Robert W. Blakesley. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers, Johannes Zschocke and Georg F. Hoffmann. Automated Fluorescent DNA Sequencing on the ABI PRISM 377, Jonathan R. E. MacBeath, Suzanna S. Harvey, and Nicola J. Oldroyd. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer, David Watts and Jonathan R. E. MacBeath. Fluorescent Sequencing Protocols for the ALF, Shirley McQuaid. Fluorescent Sequencing for Heterozygote Mutation Detection, Colin A. Graham and Alison J. M. Hill. Sequence Databases and the Internet, Anne E. Hughes. DNA Sequencing by Capillary Array Electrophoresis, Norman J. Dovichi and JianZhong Zhang. Index.