Cryopreservation and Freeze-Drying Protocols

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This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.

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InhaltsangabePART I: Fundamental Aspects And Freezing Technology                                              1          Principles of Cryopreservation                                                                   David E. Pegg  2          Principles of Cryopreservation By Vitrification                                                         Gregory M. Fahy and Brian Wowk  3          Modeling and Optimization of Cryopreservation                                                       James Benson  4          The Principles of Freeze-Drying                                                                                 Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward  PART II: Methods To Study Freezing and Drying Processes                                          5.         Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cells                                                                                                     Willem F. Wolkers and Harriëtte Oldenhof  6.         Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations           Wendell Q. Sun  7.         Measurement Of Intracellular Ice Formation Kinetics By High-Speed  Video Cryomicroscopy                                                                                          Jens O. M. Karlsson  8.         Laser Scanning Microscopy in Cryobiology                                                  Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann  9.         Low Temperature Electron Microscopy                                                                     Roland A. Fleck  PART III: Cryopreservation Protocols                                                                              10        Cryopreservation of Semen From Domestic Livestock                                              Harald Sieme, Harriëtte Oldenhof  11.       Cryopreservation of Mammalian Oocytes                                                                  Victoria Keros, Barry J. Fuller  12.       Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts                                                                                                     Juergen Liebermann  13        Efficient Cryopreservation of Human Pluripotent Stem Cells By Surface-Based Vitrification                                                                                        Julia C. Neubauer, Axel F. Beier, Nils Geijsen, and Heiko Zimmermann  14        Cryopreservation Of Greenshell Mussel (Perna Canaliculus) Sperm                      Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay T. Mcgowan, and H. Robin Tervit  15        Membrane Modification Strategies for Cryopreservation                              Phillip H. Purdy and James K. Graham  16.       Sperm Clean-Up and Density Centrifugation Procedures                              Harald Sieme and Harriëtte Oldenhof  17.       Cryopreservation of Red Blood Cells                                                                        Johan W. Lagerberg  18.       Cord Blood Clinical Processing, Cryopreservation,  and Storage                  Heidi Elmoazzen and Jelena L. Holovati  19.       Directional Freezing For Large Volume Cryopreservation                                         Joseph Saragusty  20        Vitrification Of Heart Valve Tissues                                                              Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell  21        Cryopreservation of Plant Cell Lines                     

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