Advanced Protocols in Oxidative Stress III

Zusatztext

Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.

Autorenportrait

InhaltsangabePart I: Reactive Oxygen and  Nitrogen Techniques 1. Derivatization and Detection of Small Aliphatic and Lipid-Bound Carbonylated Lipid Peroxidation Products by ESI-MS            Ivana Milic and Maria Fedorova 2. Specific Imaging and Tracking of Mitochondria in Live Cells by a Photostable AIE Luminogen            Chris W.T. Leung, Yuning Hong, and Ben Zhong Tang 3. Analysis of Relationship Between Oxidized Phospholipid Structure and Interaction with the Class B Scavenger Receptors            Detao Gao, Larry W. Sayre, and Eugene A. Podrez 4. A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA            Takayuki Shibamoto 5. Nitric Oxide Availability as a Marker of Oxidative Stress            Dan Pierini and Nathan S. Bryan 6. Quantification of DNA Repair Capacity Towards Oxidatively Damaged DNA in Subcellular and Cellular Systems by a Non-Radioactive Cleavage Assay            Ingrit Hamann and Andrea Hartwig 7. Chiral Phase-HPLC Separation of Hydroperoxyoctadecenoic Acids and Their Biosynthesis by Fatty Acid Dioxygenases            Ernst H. Oliw and Anneli Wennman 8. Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and H2S Repair            Zhangrong Lou, Peng Li, and Keli Han 9. Evaluation of Physical Integrity of Lipid Bilayer Under Oxidative Stress: Application of Fluorescence Microscopy and Digital Image Processing            Ran Liang, Jian-Ping Zhang, and Leif H. Skibsted 10. Electrochemical Detection of Glutathione S-Transferase: An Important Enzyme in the Cell Protective Mechanism Against Oxidative Stress            Manuel C. Martos-Maldonado, Juan M. Casas-Solvas, Antonio Vargas-Berenguel, and Luis García-Fuentes 11. Using Nanosensors for In Situ Monitoring and Measurement of Nitric Oxide and Peroxynitrite in a Single Cell            Tadeusz Malinski Part II: Antioxidant Technology and Application 12. Curcuma longa Attenuates Carbon Tetrachloride-Induced Oxidative Stress in T Lymphocyte Subpopulations            H.A. Abu-Rizq, Mohamed H. Mansour, and Mohammad Afzal 13. Prolongation of the Lag Time Preceding Peroxidation of Serum Lipids: A Measure of Antioxidant Capacity            Ilya Pinchuk and Dov Lichtenberg 14. Identification of (Anti-Oxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements            Eric Deconinck, Deborah Custers, and Jacques Omer De Beer 15. Simultaneous Determination of Ascorbic Acid, Aminothiols, and Methionine in Biological Matrices Using Ion-Pairing RP-HPLC Coupled with Electrochemical Detector            Muhammad Imran Khan, Zafar Iqbal, and Abad Khan 16. Portable Nanoparticle Based Sensors for Antioxidant Analysis            Erica Sharpe and Silvana Andreescu 17. Fe3+-Fe2+ Transformation Method: An Important Antioxidant Assay            Ilhami Gülçin 18. Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity            Augustin C. Mot, Cristina Bischin, Grigore Damian, and Radu Silaghi-Dumitrescu 19. Computational Studies on Conformation, Electron Density Distributions, and Antioxidant Properties of Anthocyanidins            Ricardo A. Mosquera, Laura Estévez, and Mercedes García-Bugarín 20. Automatic Flow Injection Analysis (FIA) Determination of Total Reducing Capacity in Serum and Urine Samples            Marcela A. Segundo, Ildikó V. Tóth, Luís M. Magalhães, and Salette Reis 21. Mass Spectrometry Detection of Isolevuglandin Adduction to Specific Protein Resi

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